-
Product Name
Anti-sars2 antibody
- Documents
-
Description
Rabbit polyclonal antibody to sars2
-
Tested applications
WB, IF, IHC-P
-
Species reactivity
Zebrafish
-
Alternative names
SerRS2 antibody; zgc:66345 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antigen of this antibody was recombinant protein within zebrafish sars2 aa 293-514.
-
Clonality
Polyclonal
-
Formulation
Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
-
Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
-
Applications
WB: 1:500
IF: 1:50-1:200
IHC-P: 1:50-1:200
-
Validations
Fig1:; Western blot analysis of SARS2 on Zebrafish tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2:; IF staining of SARS2 in Zebrafish (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3:; Immunohistochemical analysis of paraffin-embedded Zebrafish tissue using anti-SARS2 antibody. Counter stained with hematoxylin.
- Background
-
References
- Gaudet P et al. Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12:449-462 (2011).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"