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Product Name
Anti-S6K1 Rabbit antibody
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Description
S6K1 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP, FC
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human p70 S6 Kinase
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000-1/2000
IHC: 1/50-1/200
ICC/IF: 1/100-1/500
IP: 1/50
FC: 1/100
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Validations
Western blot analysis of p70 S6 Kinase expression in 293T cell lysate.
Western blot analysis of p70 S6 Kinase expression in SHSY-5Y,A549,HUVEC,C6 and Hela cell lysates using p70 S6 Kinase antibody at 1/1000 dilution.Predicted band size:70KDa.Observed band size:70KDa.
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Background
Swiss-Prot Acc.P23443.p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression.Phosphorylation of Thr229 in the catalytic domain and Thr389 in the linker domain are most critical for kinase function. Phosphorylation of Thr389, however, most closely correlates with p70 kinase activity in vivo. Prior phosphorylation of Thr389 is required for the action of phosphoinositide 3-dependent protein kinase 1 (PDK1) on Thr229.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"