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Product Name
Anti-S100A3 antibody
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Description
Rabbit polyclonal to S100A3
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Tested applications
ELISA, IHC-P
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Species reactivity
Human S100A3
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Alternative names
S100a3 antibody; S100A3 antibody; S100E antibody; S100E antibody; S100E antibody; S100E antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human S100A3 (rh S100A3; P33764; Met 1-Gln 101). S100A3 specific IgG was purified by Human S100A3 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human S100A3. The detection limit for Human S100A3 is approximately 0.00245 ng/well.
IHC-P: 0.5-2 μg/mL
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Validations
S100A3 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human S100A3 in human skinl with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). The image showing positive staining in hair follicle and sebaceous gland.
S100A3 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human S100A3 in human tonsil with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). The image showing positive staining in tonsil crypt.
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Background
Protein S100-A3, also known as Protein S-100E, S100 calcium-binding protein A3, S100A3 and S100E, is a member of the S-100 family. S100A3 / S100E contains 2 EF-hand domains. S100A3 / S100E is highly expressed in the differentiating cuticular cells within the hair follicle and organized into mature hair cuticles. High concentrations of S100A3 homotetramer might provide the millimolar level of Ca2+ required for hair cuticular barrier formation. S100A3 / S100E is a unique member of the Ca2+-binding S100 protein family with the highest cysteine content and affinity for Zn2+. S100A3 / S100E binds both calcium and zinc. S100A3 / S100E probably binds 2 zinc ions per molecule. It may be involved in calcium-dependent cuticle cell differentiation and hair shaft formation. S100A3 plays an important role in calcium-dependent processes leading to hair shaft formation. S100A3 / S100E is a unique protein among all members of the calcium-binding S100 family, is specifically expressed at the inner endocuticle of human hair fibers. Upon hair damage, S100A3 / S100E is released from hair fibers and possibly destabilizes the hair tissue architecture.
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References
- Kizawa, K. et al., 2008, J Biol Chem. 283 (8):5004-13.
- Kizawa, K. et al., 1998, J Invest Dermatol. 111 (5):879-86.
- Kizawa, K. et al., 2002, Biochem Biophys Res Commun. 299 (5):857-62.
- Fritz,G. et al., 2002, J Biol Chem. 277 (36):33092-8.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"