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Product Name
Anti-RPRD1B antibody
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Description
Rabbit polyclonal to RPRD1B
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Tested applications
WB, IHC-P, IP
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Species reactivity
Human RPRD1B
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Alternative names
regulation of nuclear pre-mRNA domain containing 1B antibody; RPRD1B antibody; RP5-1057B20.2 antibody; C20orf77 antibody; CREPT antibody; NET60 antibody; dJ1057B20.2 antibody; cell cycle-related and expression-elevated protein in tumor antibody; regulation of nuclear pre-mRNA domain-containing protein 1B antibody; regulation of nuclear pre-mRNA domain containing 1B antibody; Rprd1b antibody; RP23-396G1.1 antibody; 2610304G08Rik antibody; 2810446G03Rik antibody; Crept antibody; cell cycle-related and expression-elevated protein in tumor antibody; regulation of nuclear pre-mRNA domain-containing protein 1B antibody; CREPT antibody; NET60 antibody; C20orf77 antibody; dJ1057B20.2 antibody; Crept antibody; 2610304G08Rik antibody; 2810446G03Rik antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human RPRD1B (rh RPRD1B; AAH33629.1; Met1-Asp326). RPRD1B specific IgG was purified by Human RPRD1B affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 2-10 μg/mL
IHC-P: 0.05-0.2 μg/mL
IP: 1-4 μg/mg of lysate
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Validations
RPRD1B Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
RPRD1B Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human RPRD1B in human colon carcinoma with rabbit polyclonal antibody (0.1 µg/mL, formalin-fixed paraffin embedded sections).
RPRD1B Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human RPRD1B in human hepatoma with rabbit polyclonal antibody (0.1 µg/mL, formalin-fixed paraffin embedded sections).
RPRD1B Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
RPRD1B Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
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Background
RPRD1B, together with RPRD1A, can accompany RNAP II from promoter regions to 3'-untranslated regions during transcription in vivo, predominantly interact with phosphorylated RNAP II, and can reduce CTD S5- and S7-phosphorylated RNAP II at target gene promoters. RNA polymerase II C-terminal domain (CTD) phosphorylation is important for various transcription-related processes. RPRD1B is a transcriptional regulator which enhances expression of CCND1. It also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A.
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References
- Ni Z. et al., 2011, Transcription. 2 (5): 237-42.
- Kristensen AR. et al., 2012, Nat Methods. 9 (9): 907-9.
- Woods NT. et al., 2012, Sci Signal.5 (242): rs6.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"