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Product Name
Anti-RPA70 (8C3) Mouse antibody
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Description
RPA70 (8C3) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF, IP
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Species reactivity
Human, Monkey, Mouse, Rat
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Alternative names
Dmrpa1;Drosophila Replication Protein A;DRPA;DSSB;HSSB;Human single stranded DNA binding protein;MST075;MSTP075;p70;REPA 1;REPA1;Replication factor A;Replication factor A protein 1;Replication protein A 70 kDa DNA-binding subunit;Replication protein A 70k antibody
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Isotype
Mouse IgG2a
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Preparation
Antigen: Purified recombinant human RPA70 protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
;ICC: 1/100;Other 1/1000
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Validations
Immunoprecipitation analysis of Hela cell lysates using RPA70 mouse mAb.
Western blot detection of RPA70 in Hela,A431,MCF7,COS7,HT1080 and K562 cell lysates using RPA70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.
Western blot detection of RPA70 in HUVEC,Hacat,3T3,MCF7 and A549 cell lysates using RPA70 mouse mAb (1:2000 diluted).Predicted band size:70KDa.Observed band size:70KDa.
Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and using RPA70 mouse mAb (dilution 1:100).
Western blot detection of RPA70 in Hela,293T,C6,3T3 and K562 cell lysates using RPA70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.
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Background
Swiss-Prot Acc.P27694.Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"