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Product Name
Anti-Rad51 Rabbit antibody
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Description
Rad51 Rabbit polyclonal antibody
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Tested applications
WB, ICC/IF, FC, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
RECA; BRCC5; FANCR; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human Rad51
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/2000-1/10000
ICC/IF: 1/200
FC: 1/20-1/100
IP: 1/20
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Validations
Western blot detection of Rad51 in C6,3T3,Hela cell lysates using Rad51 Rabbit pAb(1:1000 diluted).Predicted band size:37kDa.Observed band size:37kDa.
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Background
Swiss-Prot Acc.Q06609.Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR) (PubMed:28575658). Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. Also involved in interstrand cross-link repair (PubMed:26253028).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"