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Product Name
Anti-RACK1-GNB2L1 antibody
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Description
Rabbit polyclonal to RACK1-GNB2L1
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Tested applications
ELISA, IHC-P
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Species reactivity
Human RACK1 / GNB2L1
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Alternative names
activated protein kinase C receptor antibody; beta 2 antibody; H12.3 antibody; beta polypeptide 2 like 1 sequence 1 antibody; HLC-7 antibody; PIG21 antibody; RACK1 antibody; Gnb2-rs1 antibody; p205 antibody; Rack1 antibody; GB-like antibody; AL033335 antibody; beta polypeptide 2-like 1 antibody; Gnb2-rs1 antibody; cell proliferation-inducing gene 21 protein antibody; GB-like antibody; Gnb2-rs1 antibody; Gnb2-rs1 antibody; guanine nucleotide binding antibody; guanine nucleotide binding protein antibody; guanine nucleotide binding protein (G protein) antibody; guanine nucleotide binding protein related antibody; guanine nucleotide-binding protein subunit beta-2-like 1 antibody; guanine nucleotide-binding protein subunit beta-2-like 1 antibody; guanine nucleotide-binding protein subunit beta-like protein 12.3 antibody; H12.3 antibody; HLC7 antibody; HLC-7 antibody; human lung cancer oncogene 7 protein antibody; lung cancer oncogene 7 antibody; PIG21 antibody; proliferation-inducing gene 21 antibody; protein homologous to chicken B complex protein antibody; Rack1 antibody; RACK1 antibody; receptor for activated C kinase antibody; Receptor for Activated C Kinase 1 antibody; receptor of activated protein kinase C 1 antibody; receptor of activated protein kinase C 1 antibody; related sequence 1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human RACK1 / GNB2L1 (rh RACK1 / GNB2L1; P63244; Met1-Arg317). RACK1 / GNB2L1 specific IgG was purified by Human RACK1 / GNB2L1 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human RACK1 / GNB2L1. The detection limit for Human RACK1 / GNB2L1 is approximately 0.00975 ng/well.
IHC-P: 0.1-2 μg/mL
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Validations
RACK1 / GNB2L1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human GNB2L1 in human hepatoma (from 2 donors) with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
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Background
RACK1 (guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1), also known as GNB2L1, contains 7 WD repeats and belongs to the WD repeat G protein beta family. In the liver, RACK1 is expressed at higher levels in activated hepatic stellate cells than in hepatocytes or Kupffer cells. It is up-regulated in hepatocellular carcinomas and in the adjacent non-tumor liver tissue. RACK1 is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. It interacts with a wide variety of proteins and plays a role in many cellular processes. GNB2L1 binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. RACK1 may recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. It inhibits the activity of SRC kinases including SRC, LCK and YES1. RACK1 also inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. It enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer.
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References
- Jannot G, et al.. (2011) The ribosomal protein RACK1 is required for microRNA function in both C. elegans and humans. EMBO Rep. 12(6):581-6.
- Wang F, et al. (2011) RACK1 regulates VEGF/Flt1-mediated cell migration via activation of a PI3K/Akt pathway. J Biol Chem. 286(11):9097-106.
- Cao XX, et al. (2011) RACK1 promotes breast carcinoma migration/metastasis via activation of the RhoA/Rho kinase pathway. Breast Cancer Res Treat. 126(3):555-63.
- Myklebust LM, et al. (2011) Receptor for activated protein C kinase 1 (RACK1) is overexpressed in papillary thyroid carcinoma. Thyroid. 21(11):1217-25.
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