Anti-PTGER4 antibody

Cat.#: 175401

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Product Information

  • Product Name
    Anti-PTGER4 antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to PTGER4
  • Tested applications
    WB, FC
  • Species reactivity
    Human, Bee
  • Alternative names
    EP4 antibody; EP4R antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was klh conjugated synthetic peptide derived from human ep4r/prostaglandin e receptor ep4 151-250/488
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
  • Storage instructions
    Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
  • Applications

    WB:1:500-2000

    FC:1μg/Test

  • Validations

    Fig1: Sample:; Raji(Human) Cell Lysate at 30 ug; Primary: Anti-EP4 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 53 kD; Observed band size: 53 kD

    Fig1: Sample:; Raji(Human) Cell Lysate at 30 ug; Primary: Anti-EP4 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 53 kD; Observed band size: 53 kD

    Fig2: Blank control (blue line): Hela (fixed with 70% methanol (Overnight at -20℃) and then permeabilized with ice-cold 90% methanol for 30 min on ice).; Primary Antibody (green line): Rabbit Anti-EP4 antibody ,Dilution: 1μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG .; Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.

    Fig2: Blank control (blue line): Hela (fixed with 70% methanol (Overnight at -20℃) and then permeabilized with ice-cold 90% methanol for 30 min on ice).; Primary Antibody (green line): Rabbit Anti-EP4 antibody ,Dilution: 1μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG .; Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.

    Fig3: Sample:; MCF-7 Cell (Human) Lysate at 30 ug; Primary: Anti-EP4 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 53 kD; Observed band size: 53 kD

    Fig3: Sample:; MCF-7 Cell (Human) Lysate at 30 ug; Primary: Anti-EP4 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 53 kD; Observed band size: 53 kD

  • Background

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"