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Product Name
Anti-Profilin 2 / PFN2 antibody
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Description
Rabbit polyclonal to Profilin 2 / PFN2
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Tested applications
ELISA, WB, IHC-P
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Species reactivity
Human Profilin 2 / PFN2
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Alternative names
profilin 2 antibody; PFN2 antibody; D3S1319E antibody; PFL antibody; profilin II antibody; profilin 2 antibody; Pfn2 antibody; Pfn antibody; profilin II antibody; Pfn antibody; PFL antibody; D3S1319E antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human Profilin 2 / PFN2 (rh Profilin 2 / PFN2; AAH18049.1; Met1-Phe140). Profilin 2 / PFN2 specific IgG was purified by Human Profilin 2 / PFN2 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human Profilin 2 / PFN2. The detection limit for Human Profilin 2 / PFN2 is < 0.039 ng/well.
IHC-P: 0.05-0.5 μg/ml
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Validations
Profilin 2 / PFN2 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
Profilin 2 / PFN2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Profilin 2 / PFN2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
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Background
Profilin 2, also known as PFN2, is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is highly expressed in brain, skeletal muscle and kidney and less strongly in heart, placenta, lung and liver. Profilin 2 binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. Profilin 2 is thought to regulate actin polymerization in response to extracellular signals. It inhibits the formation of IP3 and DG by binding to PIP2.
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References
- Da Silva. et al., 2003, J Cell Biol. 162 (7): 1267-79.
- Honore B. et al., 1993, FEBS Lett. 330 (2): 151-5.
- Joensuu T. et al., 1997, Genomics. 38 (3): 255-63.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"