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Product Name
Anti-PRK2 (4C2) Mouse antibody
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Description
PRK2 (4C2) Mouse monoclonal antibody
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Tested applications
WB, IHC-P, FC
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Species reactivity
Human, Monkey, Mouse, Rat
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human PRK2 expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Western blot detection of PRK2 in HUVEC,A549,Jurkat,K562 and Hela cell lysates using PRK2 mouse mAb (1:500 diluted).Predicted band size:140KDa.Observed band size:140KDa.
Flow cytometric analysis of NIH/3T3 cells using PRK2 mouse mAb (blue) and negative control (red).
Immunohistochemical analysis of paraffin-embedded lung cancer tissues using PRK2 mouse mAb with DAB staining.
Western blot analysis using PRK2 mouse mAb against PC-12 (1), Cos7 (2), K562 (3), Jurkat (4), Hela (5), A431 (6), C6 (7), NIH/3T3 (8) and HEK293 (9) cell lysate.
Immunohistochemical analysis of paraffin-embedded prostate tissues using PRK2 mouse mAb with DAB staining.
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Background
Swiss-Prot Acc.Q16513.Protein-kinase-C-related kinases (PRKs) are part of the lipid-regulated protein kinases (PKC) which also include liver PAK & PKN. Human PRK1 and PRK2 share structurally similar catalytic domains, but less similar N-terminal regulatory regions suggesting different regulatory domain functions. PRK1 and PRK2, as well as a third member of this family, PRK3, show distinct patterns of expression in adult tissues. Additionally, the serine-threonine kinase PRK2 can be specifically cleaved by caspase-3 (and/or caspase-3-like subfamily members) during apoptosis.
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