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Product Name
Anti-PLCG 2 Mouse antibody
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Description
PLCG 2 Mouse monoclonal antibody
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Tested applications
WB, ICC/IF, FC
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Species reactivity
Human
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Isotype
Mouse IgG2b
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Preparation
Antigen: Synthesized peptide of human PLCG2 (AA: phospho-Tyrosine 753 of human Phospholipase Cg2(cERDINSLpYDVSRMYV)).
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Clonality
Monoclonal
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Formulation
Purified antibody in PBS with 0.05% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
ICC: 1/200 - 1/1000
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Western blot analysis of extracts from Jurkat,A549 and MCF7 cell lysates using PLCγ2 mouse mAb (1:1000 diluted).Predicted band size:148KDa.Observed band size:148KDa.
Flow cytometric analysis of Hela cells using PLCG2 mouse mAb (green) and negative control (red).
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Background
Swiss-Prot Acc.P16885.The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"