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Product Name
Anti-PKR (7E7) Mouse antibody
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Description
PKR (7E7) Mouse monoclonal antibody
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Tested applications
WB, IHC-P, FC
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Species reactivity
Human
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human EIF2AK2 (AA: 329-551) expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Purified antibody in PBS with 0.05% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using EIF2AK2 mouse mAb with DAB staining.
Western blot detection of PKR in Jurkat,A549 and MCF7 cell lysates using PKR mouse mAb (1:1000 diluted).Predicted band size:62KDa.Observed band size:74KDa.
Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using EIF2AK2 mouse mAb with DAB staining.
Flow cytometric analysis of A431 cells using EIF2AK2 mouse mAb (green) and negative control (red).
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Background
Swiss-Prot Acc.P19525.The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"