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Anti-PINCH (3C12) Mouse antibody

Cat.#: 168345

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Product Information

  • Product Name
    Anti-PINCH (3C12) Mouse antibody
  • Documents
  • Description
    PINCH (3C12) Mouse monoclonal antibody
  • Tested applications
    WB, ICC/IF, FC, IP
  • Species reactivity
    Human
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant fragment of human PINCH expressed in E. Coli.
  • Clonality
    Monoclonal
  • Formulation
    Ascitic fluid containing 0.03% sodium azide.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500 - 1/2000

    ICC: 1/200 - 1/1000

    FC: 1/200 - 1/400

    ELISA: 1/10000

  • Validations

    Immunoprecipitation analysis of Hela cell lysates using PINCH mouse mAb.

    Immunoprecipitation analysis of Hela cell lysates using PINCH mouse mAb.

    Immunofluorescence analysis of HepG2 cells using PINCH mouse mAb (green). Blue

    Immunofluorescence analysis of HepG2 cells using PINCH mouse mAb (green). Blue

    Flow cytometric analysis of Hela cells using PINCH mouse mAb (blue) and negative control (red).

    Flow cytometric analysis of Hela cells using PINCH mouse mAb (blue) and negative control (red).

    Western blot detection of PINCH in C6,Jurkat,K562,3T3 and Hela cell lysates using PINCH mouse mAb (1:1000 diluted).Predicted band size:37KDa.Observed band size:35KDa.

    Western blot detection of PINCH in C6,Jurkat,K562,3T3 and Hela cell lysates using PINCH mouse mAb (1:1000 diluted).Predicted band size:37KDa.Observed band size:35KDa.

  • Background
    Swiss-Prot Acc.P48059.The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"