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Product Name
Anti-PIM1 Rabbit antibody
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Description
PIM1 Rabbit monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC
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Species reactivity
Human, Mouse
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human PIM1
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Clonality
Monoclonal
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Formulation
50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/2000-1/10000
IHC: 1/20-1/50
ICC/IF: 1/20-1/100
FC: 1/20
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Validations
Western blot detection of PIM1 in Hela,A549,HL-60 cell lysates using PIM1 Rabbit mAb(1:1000 diluted).Predicted band size:45kDa.Observed band size:34kDa.
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Background
The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).[provided by RefSeq, Aug 2011]
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"