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Product Name
Anti-Phospho-Smad3 (Ser423/425) Rabbit antibody
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Description
Phospho-Smad3 (Ser423/425) Rabbit monoclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic phosphopeptide corresponding to residues surrounding Ser423/Ser425 of human Smad3
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Clonality
Monoclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
ICC/IF: 1/50
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Validations
Western blot detection of Phospho-Smad3 (Ser423/425) in Jurkat,C6,CHO-K1,Hela cell lysates using Phospho-Smad3 (Ser423/425) Rabbit mAb(1:1000 diluted).Predicted band size:48kDa.Observed band size:52kDa.
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Background
Swiss-Prot Acc.P84022.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"