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Product Name
Anti-Phospho-Smad2 (Ser467) Rabbit antibody
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Description
Phospho-Smad2 (Ser467) Rabbit polyclonal antibody
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Isotype
IgG
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Preparation
Antigen: Peptide sequence around phosphorylation site of serine 467 (C-S-S-M-S(p)) derived from Human Smad2.
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Clonality
Polyclonal
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Formulation
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB:1/500-1/1000
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Validations
Western blot analysis of extracts from Hela cells untreated or treated with PMA using Smad2 (Phospho-Ser467) Antibody .
Western blot detection of Smad2(Phospho-Ser467) in Hela and 3T3 cells untreated or treated with Alkaline phosphatase using Smad2(Phospho-Ser467) Rabbit pAb (dilution 1:500, upper) or u03b2-Actin Mouse mAb (200068-8F10, lower).Predicted band size:60kDa.Observed band size:60kDa.
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Background
Swiss-Prot Acc.Q15796.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"