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Product Name
Anti-phospho-NDRG1 (Ser330) Rabbit antibody
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Description
phospho-NDRG1 (Ser330) Rabbit monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic phosphopeptide corresponding to residues surrounding Ser330 of human NDRG1
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Clonality
Monoclonal
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Formulation
50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/2000-1/10000
IHC: 1/50
ICC/IF: 1/20
IP: 1/20
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Validations
Western blot detection of phospho-NDRG1(Ser330) in Hela,A549,MCF-7,239T,C2C12 cell lysates using phospho-NDRG1(Ser330) Rabbit mAb(1:1000 diluted).Predicted band size:43kDa.Observed band size:43kDa.
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Background
This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
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