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Product Name
Anti-NDRG1 / CAP43 antibody
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Description
Rabbit polyclonal to NDRG1 / CAP43
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Human NDRG1 / CAP43
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Alternative names
DRG1 antibody; DRG-1 antibody; N-myc downstream regulated 1 antibody; NDRG1 antibody; CAP43 antibody; CMT4D antibody; DRG-1 antibody; DRG1 antibody; GC4 antibody; HMSNL antibody; NDR1 antibody; NMSL antibody; PROXY1 antibody; RIT42 antibody; RTP antibody; TARG1 antibody; TDD5 antibody; N-myc downstream-regulated gene 1 protein antibody; differentiation-related gene 1 protein antibody; nickel-specific induction protein Cap43 antibody; protein NDRG1 antibody; protein regulated by oxygen-1 antibody; reducing agents and tunicamycin-responsive protein antibody; N-myc downstream regulated gene 1 antibody; Ndrg1 antibody; CAP43 antibody; CMT4D antibody; DRG1 antibody; HMSNL antibody; NMSL antibody; Ndr1 antibody; Ndrl antibody; PROXY1 antibody; RTP antibody; TDD5 antibody; N-myc downstream regulated 1 antibody; N-myc downstream-regulated gene 1 protein antibody; protein NDRG1 antibody; protein Ndr1 antibody; GC4 antibody; RTP antibody; NDR1 antibody; NMSL antibody; TDD5 antibody; CAP43 antibody; CMT4D antibody; HMSNL antibody; RIT42 antibody; TARG1 antibody; PROXY1 antibody; RTP antibody; DRG1 antibody; NMSL antibody; Ndr1 antibody; Ndrl antibody; TDD5 antibody; CAP43 antibody; CMT4D antibody; HMSNL antibody; PROXY1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human NDRG1 / CAP43 (rh NDRG1 / CAP43; Q92597; Met1-Cys394). NDRG1 / CAP43 specific IgG was purified by Human NDRG1 / CAP43 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human NDRG1 / CAP43. The detection limit for Human NDRG1 / CAP43 is < 0.039 ng/well.
IHC-P: 0.05-0.5 μg/mL
IP: 1-4 μg/mg of lysate
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Validations
NDRG1 / CAP43 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
NDRG1 / CAP43 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human NDRG1 in human lung cancer with rabbit polyclonal antibody (0.05 µg/mL, formalin-fixed paraffin embedded sections).
NDRG1 / CAP43 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human NDRG1 in human colon carcinoma with rabbit polyclonal antibody (0.05 µg/mL, formalin-fixed paraffin embedded sections).
NDRG1 / CAP43 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
NDRG1 / CAP43 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
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Background
NDRG1 gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. NDRG1 is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. NDRG1 is necessary for p53-mediated caspase activation and apoptosis. Mutations in NDRG1 gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. NDRG1 is a stress-responsive protein involved in hormone responses, cell growth, and differentiation. It acts as a tumor suppressor in many cell types.
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References
- Kachhap SK, et al. (2007) The N-Myc down regulated Gene1 (NDRG1) Is a Rab4a effector involved in vesicular recycling of E-cadherin. PLoS ONE. 2(9):e844.
- Zhang J, et al. (2008) Human differentiation-related gene NDRG1 is a Myc downstream-regulated gene that is repressed by Myc on the core promoter region. Gene. 417(1-2):5-12.
- Kokame K, et al. (1997) Homocysteine-respondent genes in vascular endothelial cells identified by differential display analysis. GRP78/BiP and novel genes. J Biol Chem. 271(47): 29659-65.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"