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Product Name
Anti-Phospho-Histone H3 (Ser10) Rabbit antibody
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Description
Phospho-Histone H3 (Ser10) Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF
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Species reactivity
Human
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Isotype
Rabbit IgG
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Preparation
Antigen: Peptide sequence around phosphorylation site of serine 10 (R-K-S(p)-T-G) derived from Human Histone H3.1.
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Clonality
Polyclonal
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Formulation
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB:1/500-1/1000ICC:1/100-1/200IHC:1/50-1/100
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Validations
Immunofluorescence staining of methanol-fixed Hela cells using Histone H3.1 (Phospho-Ser10) Antibody .
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H3.1 (Phospho-Ser10) Antibody (left) or the same antibody preincubated with blocking peptide #51184 (right).
Immunofluorescent analysis of Hela cells labeled with Aurora Kinase A(200525,dilution 1:50) mouse mAb (green) and Histone H3.1 (Phospho-Ser10)(166882,dilution 1:100) Rabbit pAb (red). DAPI was used to stain nucleus(blue).
Western blot analysis of extracts from HeLa cells,untreated or 1mM hydroxyurea treated for 5 hours,using Phospho-Histone H3.1(Ser10) Rabbit pAb(1:500 diluted).
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Background
Swiss-Prot Acc.P68431.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"