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Product Name
Anti-Phospho-Histone H3 (Ser10) Rabbit antibody
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Description
Phospho-Histone H3 (Ser10) Rabbit polyclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human
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Alternative names
Histone H3/f; Histone H3; Histone H3/d; Histone H3/b; Histone H3/c; Histone H3/a; Histone H3/l; Histone H3/j; Histone H3/k; Histone H3/h; H3/A; H3FA; Histone H3/i. antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Human Phospho-Histone H3(Ser10) synthetic peptide conjugated to KLH.
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Clonality
Polyclonal
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Formulation
PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
;ICC: 1/200
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Validations
Western blot analysis of extracts from HeLa cells,untreated or 1mM hydroxyurea treated for 5 hours,using Phospho-Histone H3(Ser10) Rabbit pAb(1:1000 diluted).
Immunofluorescent analysis of Hela cells labeled with Phospho-Histone H3(Ser10) (dilution 1:200) Rabbit pAb. DAPI was used to stain nucleus(blue).
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Background
Swiss-Prot Acc.P68431.Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"