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Product Name
Anti-PGK1 Rabbit antibody
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Description
PGK1 Rabbit polyclonal antibody
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Tested applications
WB, ICC/IF, FC
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Species reactivity
Human, Mouse, Rat
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Alternative names
PGKA; MIG10; HEL-S-68p antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human PGK1
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000
ICC/IF: 1/50
FC: 1/20
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Validations
Western blot detection of PGK1 in Hela cell lysates using PGK1 Rabbit pAb(1:1000 diluted).Predicted band size:45KDa.Observed band size:45KDa.
Immunocytochemistry of PGK1(green) in Hela cells using PGK1 Rabbit pAb at dilution 1/50, and DAPI(blue)
Western blot detection of PGK1 in MCF7,HCT116,T47D,Hela cell lysates using PGK1 Rabbit pAb(1:1000 diluted).Predicted band size:45kDa.Observed band size:45kDa.
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Background
Swiss-Prot Acc.P00558.In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed:2324090). May play a role in sperm motility (PubMed:26677959).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"