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Product Name
Anti-PGK1 Rabbit antibody
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Description
PGK1 Rabbit polyclonal antibody
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Tested applications
WB, ICC/IF, FC
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human PGK1
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500-1/2000
ICC/IF: 1/50-1/200
FC: 1/50
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Validations
Western blot analysis of PGK1 expression in SKOV3,Jurkat,U251,PC3 and Hela cell lysates using PGK1 antibody at 1/1000 dilution.Predicted band size:44KDa.Observed band size:44KDa.
Western blot analysis of PGK1 expression in (1) HepG2 cell lysate; (2) Mouse kidney lysate.
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Background
Swiss-Prot Acc.P00558.The PGK1 gene encodes phosphoglycerate kinase-1, also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. It Belongs to the phosphoglycerate kinase family and defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"