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Anti-PDGFA antibody

Cat.#: 175639

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-PDGFA antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to PDGFA
  • Tested applications
    WB, FC
  • Species reactivity
    Human, Mouse, Rat, Rabbit
  • Alternative names
    PDGF1 antibody; PDGF-A antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was klh conjugated synthetic peptide derived from the missle of human pdgf-a 125-170/211
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
  • Storage instructions
    Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
  • Applications

    WB:1:500-2000

    FC:1μg/Test

  • Validations

    Fig1: Sample: A549 (human)Cell Lysate at 40 ug; Primary: Anti- PDGF-A at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 14 kD; Observed band size: 18 kD

    Fig1: Sample: A549 (human)Cell Lysate at 40 ug; Primary: Anti- PDGF-A at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 14 kD; Observed band size: 18 kD

    Fig2: Blank control (blue line): A431 cells (blue).; Primary Antibody (green line): Rabbit Anti-PDGF-A antibody ; Dilution: 1μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG .; Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC; Dilution: 1μg /test.; Protocol; The cells were fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

    Fig2: Blank control (blue line): A431 cells (blue).; Primary Antibody (green line): Rabbit Anti-PDGF-A antibody ; Dilution: 1μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG .; Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC; Dilution: 1μg /test.; Protocol; The cells were fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

    Fig3: Sample:; placenta (Mouse) Lysate at 40 ug; Primary: Anti-PDGF-A at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 14 kD; Observed band size: 17 kD

    Fig3: Sample:; placenta (Mouse) Lysate at 40 ug; Primary: Anti-PDGF-A at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 14 kD; Observed band size: 17 kD

  • Background
    P04085

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"