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Product Name
Anti-p21 Rabbit antibody
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Description
p21 Rabbit polyclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human p21
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500-1/2000
ICC/IF: 1/50-1/200
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Validations
Immunofluorescent analysis of MCF7 cells, using p21 Antibody.
Western blot analysis of p21 in (1) MCF-7 cell lysate; (2) HeLa cell lysate. (3) LnCap cell lysate; (4) U87 MG cell lysate.
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Background
Swiss-Prot Acc.P38936.The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S. However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"