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Product Name
Anti-NTRK1 antibody
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Description
Rabbit polyclonal antibody to NTRK1
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Tested applications
IHC-P, FC, WB, ICC
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Species reactivity
Human, Mouse, Rat
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Alternative names
MTC antibody; TRK antibody; TRK1 antibody; TRKA antibody; Trk-A antibody; p140-TrkA antibody
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was recombinant protein
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Clonality
Polyclonal
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Formulation
Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:500
ICC: 1:100
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Validations
Fig1: Western blot analysis of TrkA on SHSY5Y (1) and SHG-44 (2) cell lysates using anti-TrkA antibody at 1/200 dilution.
Fig2: ICC staining TrkA in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-TrkA antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-TrkA antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-TrkA antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TrkA antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of SHSY5Y cells with TrkA 1/2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
- Background
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References
- Hempstead B L et al. High-affinity NGF binding requires coexpression of the trk proto-oncogene and the low-affinity NGF receptor. Nature 350:678-683 (1991).
- Jing S et al. Nerve growth factor mediates signal transduction through trk homodimer receptors. Neuron 9:1067-1079 (1992).
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