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Product Name
Anti-NOD2 antibody
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Description
Rabbit polyclonal antibody to NOD2
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Tested applications
WB, FC
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Species reactivity
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit
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Alternative names
CD antibody; ACUG antibody; BLAU antibody; IBD1 antibody; YAOS antibody; BLAUS antibody; NLRC2 antibody; NOD2B antibody; CARD15 antibody; CLR16.3 antibody; PSORAS1 antibody
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was klh conjugated synthetic peptide derived from human card15 251-350/1040
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Clonality
Polyclonal
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Formulation
Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
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Storage instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
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Applications
WB:1:500-2000
FC:1ug/Test
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Validations
Fig1: Sample:; MCF-7(Human) Cell Lysate at 30 ug; Primary: Anti-NOD2 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 114 kD; Observed band size: 114 kD
Fig2: Sample:; A549(Human) Cell Lysate at 30 ug; MCF-7(Human) Cell Lysate at 30 ug; Primary: Anti-NOD2 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 114 kD; Observed band size: 114 kD
Fig3: Blank control(blue): Raji(fixed with 2% paraformaldehyde (10 min)).; Primary Antibody:Rabbit Anti-CARD15/AF488 Conjugated antibody(175296#-/AF488 ), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions );
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"