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Product Name
Anti-NKX3-1 antibody
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Description
Mouse monoclonal antibody to NKX3-1
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Tested applications
WB, IHC-P, FC
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Species reactivity
Human
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Alternative names
NKX3 antibody; BAPX2 antibody; NKX3A antibody; NKX3.1 antibody
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Isotype
Mouse IgG2b
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Preparation
This antigen of this antibody was recombinant protein
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Clonality
Monoclonal
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Formulation
Liquid, 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:500-1:1,000
IHC-P: 1:50-1:200
FC: 1:100-1:200
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Validations
Fig1: Western blot analysis of NKX3A on LNCaP cell lysate using anti-NKX3A antibody at 1/1,000 dilution.
Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-NKX3A antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-NKX3A antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-NKX3A antibody. Counter stained with hematoxylin.
Fig5: Flow cytometric analysis of PC-3 cells with NKX3A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).
- Background
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References
- Jiang A et al. p53 overexpression represses androgen-mediated induction of NKX3.1 in a prostate cancer cell line.Exp Mol Med 38(6):625-33 (2006).
- Zhang Y et al. Structural and functional analysis of domains mediating interaction between the bagpipe homologue, Nkx3.1 and serum response factor. Exp Biol Med (Maywood) 233(3):297-309 (2006).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"