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Product Name
Anti-MTH1 Rabbit antibody
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Description
MTH1 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC, IP
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Species reactivity
Human
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Alternative names
MTH1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human MTH1
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/2000
IHC: 1/20
ICC/IF: 1/100
FC: 1/20
IP: 1/20
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Validations
Western blot detection of MTH1 in K562,Hela cell lysates using MTH1 Rabbit pAb(1:1000 diluted).Predicted band size:23kDa.Observed band size:18kDa.
Immunocytochemistry of MTH1(green) in Hela cells using MTH1 Rabbit pAb at dilution 1/50, and DAPI(blue)
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Background
Swiss-Prot Acc.P36639.Antimutagenic (PubMed:8226881, PubMed:7713500, PubMed:10608900). Plays a redundant role in sanitizing oxidized nucleotide pools, such as 8-oxo-dGTP pools (PubMed:28679043). Acts as a sanitizing enzyme for oxidized nucleotide pools, thus suppressing cell dysfunction and death induced by oxidative stress (PubMed:12857738, PubMed:24695224, PubMed:24695225). Hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thus preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions (PubMed:8226881, PubMed:10373420, PubMed:10608900, PubMed:11756418, PubMed:12857738, PubMed:16607562, PubMed:24695224, PubMed:24695225, PubMed:26999531, PubMed:28035004). Able to hydrolyze also the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP (PubMed:10373420, PubMed:11139615). Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA .
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"