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Product Name
Anti-MMP9 Rabbit antibody
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Description
MMP9 Rabbit polyclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human MMP9
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20°C . Avoid freeze / thaw cycles.
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Applications
WB: 1/500 - 1/2000
;IHC: 1/100 - 1/200
;IF: 1/50 - 1/200
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Validations
Immunofluorescence - [KO Validated] MMP9 Polyclonal Antibody. Immunofluorescence analysis of U-2OS cells using MMP9 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot - [KO Validated] MMP9 Polyclonal Antibody.Western blot analysis of extracts of various cell lines, using MMP9 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Exposure time: 10s.
Western blot - [KO Validated] MMP9 Polyclonal Antibody.Western blot analysis of extracts from normal (control) and MMP9 knockout (KO) 293T cells, using MMP9 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Exposure time: 90s.
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Background
Swiss-Prot Acc.P14780.
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References
- The accumulation of macrophages attenuates the effect of recombinant human endostatin on lung cancer
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