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Product Name
Anti-MEK2 (5C9) Mouse antibody
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Description
MEK2 (5C9) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF, FC
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Species reactivity
Human, Mouse, Rat
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human MAP2K2 expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
ICC: 1/200 - 1/1000
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Western blot detection of MEK2 in Rat Brain,3T3,Hela,Jurkat and PC3 cell lysates using MEK2 mouse mAb (1:1000 diluted).Predicted band size:44KDa.Observed band size:45KDa.
Flow cytometric analysis of Hela cells using anti-MAP2K2 mAb (green) and negative control (purple).
Immunofluorescence analysis of Hela cells using anti-MAP2K2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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Background
Swiss-Prot Acc.P36507.MAP2K2, also called MEK2, it is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"