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Product Name
Anti-MAP2 antibody
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Description
Rabbit polyclonal to MAP2
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Mouse METAP2 / MAP2
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Alternative names
p67 antibody; MAP2 antibody; MNPEP antibody; p67eIF2 antibody; p67 antibody; Amp2 antibody; Mnpep antibody; p67eIF2 antibody; AI047573 antibody; AL024412 antibody; AU014659 antibody; 4930584B20Rik antibody; A930035J23Rik antibody; P67 antibody; Amp2 antibody; eIF-2-associated p67 antibody; eIF-2-associated p67 homolog antibody; initiation factor 2-associated 67 kDa glycoprotein antibody; initiation factor 2-associated 67 kDa glycoprotein antibody; initiation factor 2-associated protein (p67) antibody; MAP 2 antibody; MAP 2 antibody; MAP2 antibody; metAP 2 antibody; metAP 2 antibody; methionine aminopeptidase 2 antibody; Mnpep antibody; MNPEP antibody; p67 antibody; p67eIF2 antibody; p67eIF2 antibody; peptidase M 2 antibody; peptidase M 2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse METAP2 / MAP2 (rM METAP2 / MAP2; O08663; Ala2-Tyr478). METAP2 / MAP2 specific IgG was purified by Mouse METAP2 / MAP2 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse METAP2 / MAP2. The detection limit for Mouse METAP2 / MAP2 is approximately 0.039 ng/well.
IHC-P: 0.1-2 μg/mL
IP: 1-4 μg/mg of lysate
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Validations
METAP2 / MAP2 Antibody, Rabbit PAb, Antigen Affinity Purified
METAP2 / MAP2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse METAP2 in mouse kidney with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
METAP2 / MAP2 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
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Background
METAP2 (Methionine aminopeptidase 2), also known as MAP2 is a a protein which belongs to the peptidase M24A family. MAP2 binds 2 cobalt or manganese ions and contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. It is found in all organisms and is especially important because of its critical role in tissue repair and protein degradation. The catalytic activity of human MAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. MAP2 protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. It also plays a critical role in the regulation of protein synthesis.
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References
- Bennett, et al. (1997) EPR Studies on the Mono- and Dicobalt (II)-Substituted Forms of the Aminopeptidase from Aeromonas proteolytica. Insight into the Catalytic Mechanism of Dinuclear Hydrolases. J Am Chem Soc. 119:1923-33.
- Johansson, et al. (2008) Dicobalt II-II, II-III, and III-III Complexes as Spectroscopic Models for Dicobalt Enzyme Active Sites. Inorg Chem. 47:5079-92.
- Bradshaw, et al. (2002) Aminopeptidases and angiogenesis. Essays Biochem. 38: 5-78.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"