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Product Name
Anti-MAP1LC3A Rabbit antibody
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Description
MAP1LC3A Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human MAP1LC3A
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/2000
IHC: 1/20
ICC/IF: 1/20-1/100
FC: 1/20-1/100
IP: 1/20
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Validations
Western blot detection of MAP1LC3A in K562,Rat Brain,Hela cell lysates using MAP1LC3A Rabbit pAb(1:1000 diluted).Predicted band size:14kDa.Observed band size:16kDa.
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Background
Swiss-Prot Acc.Q9H492.Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20713600, PubMed:24290141). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20713600).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"