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Anti-malE antibody

Cat.#: 176511

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-malE antibody
  • Documents
  • Description
    Rabbit monoclonal antibody to malE
  • Tested applications
    WB, IP
  • Species reactivity
    E.coli
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was synthetic peptide within escherichia coli mbp aa 330-370.
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:1,000-1:5,000

    IP: 2-5 µg/ml.

  • Validations

    Fig1:; Western blot analysis of Maltose Binding Protein on recombinant MBP-tag protein lysates using anti-Maltose Binding Protein antibody at 1/1,000 dilution.

    Fig1:; Western blot analysis of Maltose Binding Protein on recombinant MBP-tag protein lysates using anti-Maltose Binding Protein antibody at 1/1,000 dilution.

    Fig2:; MBP tag was immunoprecipitated in 2µg MBP Tag fusion protein lysate with 176511# at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using 176511# at 1/1,000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 60 mins at room temperature.; Lane 1: MBP Tag fusion protein lysate (input).; Lane 2: 176511# IP in MBP Tag fusion protein lysate.; Blocking/Dilution buffer: 5% NFDM/TBST

    Fig2:; MBP tag was immunoprecipitated in 2µg MBP Tag fusion protein lysate with 176511# at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using 176511# at 1/1,000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 60 mins at room temperature.; Lane 1: MBP Tag fusion protein lysate (input).; Lane 2: 176511# IP in MBP Tag fusion protein lysate.; Blocking/Dilution buffer: 5% NFDM/TBST

  • Background
    P0AEX9
  • References
    • Ishida K et al. Immunolocalization of anti-hsf1 to the acetabular glands of infectious schistosomes suggests a non-transcriptional function for this transcriptional activator. PLoS Negl Trop Dis 8:e3051 (2014).
    • Beck J et al. Ubiquitylation-dependent localization of PLK1 in mitosis. Nat Cell Biol 15:430-9 (2013).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"