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Product Name
Anti-LYRIC/AEG1 (2E4) Mouse antibody
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Description
LYRIC/AEG1 (2E4) Mouse monoclonal antibody
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Tested applications
WB, IHC-P, FC
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Species reactivity
Human
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human Metadherin expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Immunohistochemical analysis of paraffin-embedded human Liver tissues using Metadherin mouse mAb
Western blot detection of Lyric/Metadherin in Jurkat,A549,MCF7 and Hela cell lysates using Lyric/Metadherin mouse mAb (1:5000 diluted).Predicted band size:64KDa.Observed band size:75KDa.
Flow cytometric analysis of Hela cells using Metadherin mouse mAb (green) and negative control (purple).
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Background
Swiss-Prot Acc.Q86UE4.Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain; blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"