Anti-LYRIC/AEG1 (2E4) Mouse antibody

Cat.#: 168492

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Product Information

  • Product Name
    Anti-LYRIC/AEG1 (2E4) Mouse antibody
  • Documents
  • Description
    LYRIC/AEG1 (2E4) Mouse monoclonal antibody
  • Tested applications
    WB, IHC-P, FC
  • Species reactivity
    Human
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant fragment of human Metadherin expressed in E. Coli.
  • Clonality
    Monoclonal
  • Formulation
    Ascitic fluid containing 0.03% sodium azide.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500 - 1/2000

    IHC: 1/200 - 1/1000

    FC: 1/200 - 1/400

    ELISA: 1/10000

  • Validations

    Immunohistochemical analysis of paraffin-embedded human Liver tissues using Metadherin mouse mAb

    Immunohistochemical analysis of paraffin-embedded human Liver tissues using Metadherin mouse mAb

    Western blot detection of Lyric/Metadherin in Jurkat,A549,MCF7 and Hela cell lysates using Lyric/Metadherin mouse mAb (1:5000 diluted).Predicted band size:64KDa.Observed band size:75KDa.

    Western blot detection of Lyric/Metadherin in Jurkat,A549,MCF7 and Hela cell lysates using Lyric/Metadherin mouse mAb (1:5000 diluted).Predicted band size:64KDa.Observed band size:75KDa.

    Flow cytometric analysis of Hela cells using Metadherin mouse mAb (green) and negative control (purple).

    Flow cytometric analysis of Hela cells using Metadherin mouse mAb (green) and negative control (purple).

  • Background
    Swiss-Prot Acc.Q86UE4.Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain; blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"