AEG-1/MTDH-Specific Rabbit Polyclonal antibody. Positive IP detected in MCF-7 cells. Positive WB detected in MCF7 cells, A375 cells, A549 cells, DU 145 cells, HeLa cells, HepG2 cells, PC-3 cells, RAW 264.7 cells. Positive IHC detected in human prostate cancer tissue, human breast cancer tissue, human liver cancer tissue, human stomach cancer tissue, mouse brain tissue. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 65-70 kDa
ELISA, WB, IHC, IF, IP
Human,Mouse,Rat; other species not tested.
3D3 antibody; 3D3/LYRIC antibody; AEG 1 antibody; AEG1 antibody; LYRIC antibody; LYRIC/3D3 antibody; metadherin antibody; Metastasis adhesion protein antibody; MTDH antibody; Protein LYRIC antibody
This antibody was obtained by immunization of AEG-1/MTDH-Specific recombinant protein (Accession Number: NM_178812). Purification method: Antigen affinity purified.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:107899(AEG-1 antibody) at dilution of 1:1000
WB result of AEG-1/MTDH antibody (Catalog No:107899, 1:2000) with si-Control and si-MTDH transfected A549 cells.
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:107899(AEG-1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:107899(AEG-1 antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:107899(AEG-1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:107899(AEG-1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using MTDH antibody Catalog No:107899 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-AEG-1 (IP:Catalog No:107899, 3ug; Detection:Catalog No:107899 1:1000) with MCF-7 cells lysate 3250ug.
MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression. This antibody got two bands about 70 kDa and 80 kDa in western blotting, possibly due to posttranslational modification. This antibody is specific to AEG-1/MTDH.
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