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AEG-1/MTDH-Specific antibody

Cat.#: 107899

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Special Price 239.0 USD

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Product Information

  • Product Name
    AEG-1/MTDH-Specific antibody
  • Documents
  • Description
    AEG-1/MTDH-Specific Rabbit Polyclonal antibody. Positive IP detected in MCF-7 cells. Positive WB detected in MCF7 cells, A375 cells, A549 cells, DU 145 cells, HeLa cells, HepG2 cells, PC-3 cells, RAW 264.7 cells. Positive IHC detected in human prostate cancer tissue, human breast cancer tissue, human liver cancer tissue, human stomach cancer tissue, mouse brain tissue. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 65-70 kDa
  • Tested applications
    ELISA, WB, IHC, IF, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    3D3 antibody; 3D3/LYRIC antibody; AEG 1 antibody; AEG1 antibody; LYRIC antibody; LYRIC/3D3 antibody; metadherin antibody; Metastasis adhesion protein antibody; MTDH antibody; Protein LYRIC antibody
  • Immunogen
    Human AEG-1 Recombinant protein (GST tag)
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of AEG-1/MTDH-Specific recombinant protein (Accession Number: NM_178812). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:107899(AEG-1 antibody) at dilution of 1:1000

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:107899(AEG-1 antibody) at dilution of 1:1000

    WB result of AEG-1/MTDH antibody (Catalog No:107899, 1:2000) with si-Control and si-MTDH transfected A549 cells.

    WB result of AEG-1/MTDH antibody (Catalog No:107899, 1:2000) with si-Control and si-MTDH transfected A549 cells.

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:107899(AEG-1 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:107899(AEG-1 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:107899(AEG-1 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:107899(AEG-1 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:107899(AEG-1 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:107899(AEG-1 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:107899(AEG-1 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:107899(AEG-1 antibody) at dilution of 1:100 (under 40x lens)

    Immunofluorescent analysis of MCF-7 cells, using MTDH antibody Catalog No:107899 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    Immunofluorescent analysis of MCF-7 cells, using MTDH antibody Catalog No:107899 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    IP Result of anti-AEG-1 (IP:Catalog No:107899, 3ug; Detection:Catalog No:107899 1:1000) with MCF-7 cells lysate 3250ug.

    IP Result of anti-AEG-1 (IP:Catalog No:107899, 3ug; Detection:Catalog No:107899 1:1000) with MCF-7 cells lysate 3250ug.

  • Background
    MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression. This antibody got two bands about 70 kDa and 80 kDa in western blotting, possibly due to posttranslational modification. This antibody is specific to AEG-1/MTDH.
  • References
    • Yu C, Liu Y, Tan H. Metadherin regulates metastasis of squamous cell carcinoma of the head and neck via AKT signalling pathway-mediated epithelial-mesenchymal transition. Cancer letters. 343(2):258-67. 2014.
    • Yang G, Zhang L, Lin S. AEG-1 is associated with tumor progression in nonmuscle-invasive bladder cancer. Medical oncology (Northwood, London, England). 31(6):986. 2014.
    • Zhu GC, Yu CY, She L. Metadherin regulation of vascular endothelial growth factor expression is dependent upon the PI3K/Akt pathway in squamous cell carcinoma of the head and neck. Medicine. 94(6):e502. 2015.
    • Yan JJ, Zhang YN, Liao JZ. MiR-497 suppresses angiogenesis and metastasis of hepatocellular carcinoma by inhibiting VEGFA and AEG-1. Oncotarget. 6(30):29527-42. 2015.
    • Tong L, Wang C, Hu X. Correlated overexpression of metadherin and SND1 in glioma cells. Biological chemistry. 397(1):57-65. 2016.
    • Song H, Li C, Li R, Geng J. Prognostic significance of AEG-1 expression in colorectal carcinoma. International journal of colorectal disease. 25(10):1201-9. 2010.
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    • Li C, Li R, Song H. Significance of AEG-1 expression in correlation with VEGF, microvessel density and clinicopathological characteristics in triple-negative breast cancer. Journal of surgical oncology. 103(2):184-92. 2011.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"