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Product Name
Anti-LOC9270361 antibody
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Description
Rabbit polyclonal antibody to LOC9270361
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Tested applications
WB, ELISA
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Species reactivity
Rice
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Alternative names
ARF16 antibody; OsJ_019622 antibody
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was synthetic peptide within japonica rice aa 580-640.
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Clonality
Polyclonal
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Formulation
Liquid, 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:500-1:1,000
ELISA: 1:5,000
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Validations
Fig1:; Western blot analysis of ARF16 on rice tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
- Background
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References
- Wang D. et. al. Genome-wide analysis of the auxin response factors (ARF) gene family in rice (Oryza sativa). Gene 394:13-24(2007).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"