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Anti-LILRB2 antibody

Cat.#: 175073

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-LILRB2 antibody
  • Documents
  • Description
    Mouse monoclonal antibody to LILRB2
  • Tested applications
    WB, FC
  • Species reactivity
    Human
  • Alternative names
    ILT4 antibody; LIR2 antibody; CD85D antibody; ILT-4 antibody; LIR-2 antibody; MIR10 antibody; MIR-10 antibody
  • Isotype
    Mouse IgG2a
  • Preparation
    This antigen of this antibody was purified recombinant fragment of human lilrb2 (aa: 51-184) expressed in e. coli.
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*PBS with 0.05% sodium azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-1:2,000

    FC: 1:100-1:200

  • Validations

    Fig1: Western blot analysis of LILRB2 against human LILRB2 (AA: 51-184) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig1: Western blot analysis of LILRB2 against human LILRB2 (AA: 51-184) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig2: Western blot analysis of 175073# against HEK293 (1) and LILRB2 (AA: 51-184)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig2: Western blot analysis of 175073# against HEK293 (1) and LILRB2 (AA: 51-184)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig3: Western blot analysis of 175073# against MOLT4 (1) and K562 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig3: Western blot analysis of 175073# against MOLT4 (1) and K562 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig4: Flow cytometric analysis of LILRB2 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody ( 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

    Fig4: Flow cytometric analysis of LILRB2 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody ( 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

  • Background
    Q8N423
  • References
    • J Immunol Res. 2016;2016:4163094.
    • PLoS One. 2014 Mar 27;9(3):e92018.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"