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Product Name
Anti-Leukotriene A4 Hydrolase/LTA4H antibody
- Documents
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Description
Rabbit monoclonal to Leukotriene A4 Hydrolase/LTA4H
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Tested applications
ELISA, WB, IF, IP, ICC/IF
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Species reactivity
Human Leukotriene A4 Hydrolase / LTA4HNo cross activity in ELISA with Mouse LTA4H
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Alternative names
Leukotriene A4 Hydrolase antibody; Leukotriene A4 Hydrolase antibody; LTA4 hydrodase antibody; Lta4h antibody; LTA4H antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Leukotriene A4 Hydrolase / LTA4H (rh Leukotriene A4 Hydrolase / LTA4H; NP_000886.1; Met 1-Asp 611).
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human LTA4H.
ICC/IF: 10-25 μg/mL
IP: 2-10 μg/mg of lysate
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Validations
Leukotriene A4 Hydrolase / LTA4H Antibody, Rabbit MAb
Immunofluorescence staining of Human LTA4H in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human LTA4H monoclonal antibody (15 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Leukotriene A4 Hydrolase / LTA4H Antibody, Rabbit MAb, Immunoprecipitation
Leukotriene A4 Hydrolase / LTA4H Antibody, Rabbit MAb, Western blot
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Background
Mouse leukotriene A-4 hydrolase, also known as LTA-4 hydrolase, Leukotriene A (4) hydrolase, LTA4H and LTA4, is cytoplasm protein which belongs to the peptidase M1 family. LTA4H hydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4). This enzyme also has some peptidase activity. The leukotrienes (LTs) are a class of structurally related lipid mediators involved in the development and maintenance of inflammatory and allergic reactions. In the biosynthesis of LTs, arachidonic acid was converted into the unstable intermediate epoxide LTA4, which may in turn be conjugated with glutathione to form the spasmogenic LTC4, or hydrolyzed into the proinflammatory lipid mediator LTB4 in a reaction catalyzed by Leukotriene A4 hydrolase (LTA4H). LTB4 is a classical chemoattractant of human neutrophils and triggers adherence and aggregation of leukocytes to vascular endothelium, and also modulates immune responses. As a bifunctional zinc metalloenzyme, LTA4H also exhibits an anion-dependant arginyl aminopeptidase activity of high efficiency and specificity in addition to its epoxide hydrolase activity. LTA4H is regarded as a therapeutic target for inflammation.
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References
- Mancini, JA. et al.,1995, Eur. J. Biochem. 231: 65-71.
- Orning, L. et al., 1994, J. Biol. Chem. 269: 11269-73.
- Rudberg, PC. et al., 2004, J. Biol. Chem. 279: 27376-82.
- Qiu, H. et al., 2006, Proc. Natl. Acad. Sci. USA. 103: 8161-6.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"