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Product Name
Anti-LEF1 Rabbit antibody
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Description
LEF1 Rabbit monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC
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Species reactivity
Human
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Alternative names
LEF-1; TCF10; TCF7L3; TCF1ALPHA antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human LEF1
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Clonality
Monoclonal
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Formulation
50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
IHC: 1/20
ICC/IF: 1/20-1/50
FC: 1/20
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Validations
Western blot detection of LEF1 in Jurkat cell lysates using LEF1 Rabbit mAb(1:1000 diluted).Predicted band size:44kDa.Observed band size:25-58kDa.
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Background
Swiss-Prot Acc.Q9UJU2.Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 . Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
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