Anti-Lamin B1 Rabbit antibody
Lamin B1 Rabbit polyclonal antibody
ICC/IF, WB, IHC-P, ELISA
Human, Mouse, Rat
Antigen: The antiserum was produced against synthesized peptide derived from human Lamin B1. AA range:26-75
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
WB: 1/500 - 1/2000
IHC: 1/100 - 1/300
Immunofluorescence analysis of rat-kidney tissue. 1,Lamin B1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot detection of Lamin B1 in T47D,3T3,COS7 and C6 cell lysates using Lamin B1 rabbit pAb (1:20000 diluted).Predicted band size:67kDa.Observed band size:68kDa.
Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,Lamin B1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
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