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Product Name
Anti-KLHL11 antibody
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Description
Mouse monoclonal antibody to KLHL11
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Tested applications
WB, ICC/IF, FC
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Species reactivity
Human
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Isotype
Mouse IgG1
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Preparation
This antigen of this antibody was recombinant protein
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Clonality
Monoclonal
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Formulation
Liquid, 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:500-1:1,000
ICC/IF: 1:50-1:200
FC: 1:50-1:200
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Validations
Fig1: Western blot analysis of KLHL11 on Hela and MCF-7 cell lysate using anti-KLHL11 antibody at 1/1,000 dilution.
Fig2: ICC staining KLHL11 (green) and Actin filaments (red) in 3T3-L1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Flow cytometric analysis of K562 cells with KLHL11 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).
- Background
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References
- Gerhard DS et al. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res. 2004 Oct;14(10B):2121-7.
- Ota T et al. Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 2004 Jan;36(1):40-5.
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