Anti-K-48 Linkage Specific Ubiquitin Rabbit antibody

Cat.#: 167195

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Product Information

  • Product Name
    Anti-K-48 Linkage Specific Ubiquitin Rabbit antibody
  • Documents
  • Description
    K-48 Linkage Specific Ubiquitin Rabbit polyclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF, FC
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    HEL-S-50 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic peptide of human K-48 Linkage Specific Ubiquitin
  • Clonality
    Polyclonal
  • Formulation
    Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
  • Storage instructions
    Store at -20°C. Stable for 12 months from date of receipt.
  • Applications

    WB: 1/2000

    IHC: 1/50

    ICC/IF: 1/100

    FC: 1/20

  • Validations

    Immunohistochemistry of K-48 Linkage Specific Ubiquitin in paraffin-embedded Human colon cancer tissue using K-48 Linkage Specific Ubiquitin Rabbit pAb at dilution 1/50

    Immunohistochemistry of K-48 Linkage Specific Ubiquitin in paraffin-embedded Human colon cancer tissue using K-48 Linkage Specific Ubiquitin Rabbit pAb at dilution 1/50

    Western blot detection of K-48 Linkage Specific Ubiquitin in 293 cell lysates using K-48 Linkage Specific Ubiquitin Rabbit pAb(1:1000 diluted).

    Western blot detection of K-48 Linkage Specific Ubiquitin in 293 cell lysates using K-48 Linkage Specific Ubiquitin Rabbit pAb(1:1000 diluted).

  • Background
    Swiss-Prot Acc.P0CG47.Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"