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  • Anti-IRAK4 Rabbit antibody

Anti-IRAK4 Rabbit antibody

Cat.#: 168985

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Product Information

  • Product Name
    Anti-IRAK4 Rabbit antibody
  • Documents
  • Description
    IRAK4 Rabbit monoclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF, FC
  • Species reactivity
    Human
  • Alternative names
    IPD1; REN64; IRAK-4; NY-REN-64 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic peptide of human IRAK4
  • Clonality
    Monoclonal antibody
  • Formulation
    50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000-1/5000

    IHC: 1/20-1/500

    ICC/IF: 1/20

    FC: 1/20

  • Validations

    Western blot detection of IRAK4 in Jurkat,Hela cell lysates using IRAK4 Rabbit mAb(1:1000 diluted).Predicted band size:52kDa.Observed band size:52kDa.

    Western blot detection of IRAK4 in Jurkat,Hela cell lysates using IRAK4 Rabbit mAb(1:1000 diluted).Predicted band size:52kDa.Observed band size:52kDa.

  • Background
    Swiss-Prot Acc.Q9NWZ3.Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed:17878374). Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"