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Product Name
Anti-Integrin beta 1 (1G9) Mouse antibody
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Description
Integrin beta 1 (1G9) Mouse monoclonal antibody
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Tested applications
WB, IHC-P, FC
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Species reactivity
Human, Monkey
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human ITGB1 expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Purified antibody in PBS with 0.05% sodium azide
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using ITGB1 mouse mAb with DAB staining.
Western blot detection of Integrin u03b21 in C6uff0cJurkat and Hela cell lysates using Integrin u03b21 mouse mAb (1:1000 diluted).Predicted band size:88KDa.Observed band size:115,135KDa.
Flow cytometric analysis of Hela cells using ITGB1 mouse mAb (green) and negative control (red).
Western blot analysis using ITGB1 mouse mAb against Hela (1), HepG2 (2), A549 (3), Jurkat(4), L1210 (5) and Cos7 (6) cell lysate.
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ITGB1 mouse mAb with DAB staining.
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Background
Swiss-Prot Acc.P05556.Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"