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Product Name
Anti-Igsf8 antibody
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Description
Rabbit polyclonal antibody to Igsf8
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Tested applications
WB, FC
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Species reactivity
Human, Mouse, Rat, Pig, Cow, Horse
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Alternative names
P antibody; EWI- antibody; PGRL antibody; ESTM3 antibody; EWI-2 antibody; KCT-4 antibody; ESTM34 antibody
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was klh conjugated synthetic peptide derived from mouse igsf8 101-200/611
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Clonality
Polyclonal
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Formulation
Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
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Storage instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
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Applications
WB:1:500-2000
FC:1ug/Test
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Validations
Fig1: Blank control(blue):Raji (fixed with 2% paraformaldehyde (10 min)).; Primary Antibody:Rabbit Anti-IGSF8 antibody , Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;; Isotype Control Antibody: Rabbit IgG(orange), used under the same conditions );; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Fig2: Sample: Brain(Mouse) lysate at 30ug;; Primary: Anti-IGSF8 at 1:300 dilution;; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution ;; Predicted band size: 62 kD; Observed band size: 55 kD
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"