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Anti-IGHG1 antibody

Cat.#: 176630

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-IGHG1 antibody
  • Documents
  • Description
    Mouse monoclonal antibody to IGHG1
  • Tested applications
    WB, ELISA
  • Species reactivity
    Human
  • Isotype
    Mouse IgG
  • Preparation
    This antigen of this antibody was full length native protein corresponding to human igg.
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*PBS (pH7.4).
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-1:2,000

    ELISA: 1:1,000-1:20,000

  • Validations

    Fig1:; Western blot analysis of Mouse anti-Human IgG on Human IgG protein. Proteins were transferred to a PVDF membrane and blocked with 5% NFTM/TBST in PBS for 1 hour at room temperature. The primary antibody ( 1ug/ml) was used in 5% NFTM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.; Positive control:; Lane 1: Human IgG protein; Lane 2: Human IgM protein; This antibody does not cross-react with the human IgM protein.

    Fig1:; Western blot analysis of Mouse anti-Human IgG on Human IgG protein. Proteins were transferred to a PVDF membrane and blocked with 5% NFTM/TBST in PBS for 1 hour at room temperature. The primary antibody ( 1ug/ml) was used in 5% NFTM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.; Positive control:; Lane 1: Human IgG protein; Lane 2: Human IgM protein; This antibody does not cross-react with the human IgM protein.

    Fig2:; Immobilized Human IgG protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody for 1 hour at 25℃.

    Fig2:; Immobilized Human IgG protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody for 1 hour at 25℃.

  • Background
    P01857

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"