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Product Name
Anti-HTR1A antibody
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Description
Rabbit polyclonal antibody to HTR1A
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Tested applications
WB, IHC-P
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Species reactivity
Human, Mouse, Rat, Dog, Horse, Rabbit
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Alternative names
G-21 antibody; 5HT1a antibody; PFMCD antibody; 5-HT1A antibody; 5-HT-1A antibody; ADRBRL1 antibody; ADRB2RL1 antibody
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was klh conjugated synthetic peptide derived from human 5-htr1a 301-400/422
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Clonality
Polyclonal
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Formulation
Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
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Storage instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
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Applications
WB:1:500-2000
IHC-P:1:400-800
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Validations
Fig1:; Protein: HepG2 lyates(hu);; Primary: Rabbit Anti- 5HT1A Receptor at 1:300;; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000;; Predicted band size:46 kD Observed band size:56 kD
Fig2:; Protein: Brain(Mouse) Lysate at 40 ug; Primary: Rabbit Anti- 5HT1A Receptor at 1:300;; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000;; Predicted band size:46 kD Observed band size:56 kD
Fig3:; Sample:; Jurkat(Human) Cell Lysate at 40 ug; Primary: Anti-5HT1A Receptor at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 46 kD; Observed band size: 46 kD
- Background
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References
- Penington N.J., Kelly J.S. Serotonin receptor activation reduces calcium current in an acutely dissociated adult central neuron. Neuron 4:751-758(1990)
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