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Product Name
Anti-HMGB1 Rabbit antibody
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Description
HMGB1 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human HMGB1
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000-1/2000
IHC: 1/50-1/100
ICC/IF: 1/50-1/100
FC: 1/30
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Validations
Immunofluorescent analysis of SW480 cells, using HMGB1 Antibody.
Western blot analysis of HMGB1 expression in (1)HeLa cell lysate; (2)Rat brain lysate.
Western blot analysis of extracts from HCT116, MCF7 and Jurkat cells using HMGB1 Rabbit pAb at 1:1000 dilution. Predicted band size: 25kDa. Observed band size: 25kDa.
Immunohistochemical analysis of paraffin-embedded human kidney, using HMGB1 Antibody.
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Background
Swiss-Prot Acc.P09429.High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin. Evidence suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture including DNA bending and unwinding of the helix. HMG proteins synergize with Oct-2, members of the NFκB family, ATF-2 and c-Jun to activate transcription.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"