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Anti-HIF3A antibody

Cat.#: 175497

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-HIF3A antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to HIF3A
  • Tested applications
    WB, IHC-P, FC
  • Species reactivity
    Human, Mouse, Rat, Dog, Pig, Cow, Horse
  • Alternative names
    IPAS antibody; MOP7 antibody; PASD7 antibody; HIF-3A antibody; bHLHe17 antibody; HIF3-alpha-1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was klh conjugated synthetic peptide derived from human hif3 alpha 131-230/669
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
  • Storage instructions
    Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
  • Applications

    WB:1:500-2000

    IHC-P:1:400-800

    FC:1μg/Test

  • Validations

    Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HIF3 alpha) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

    Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HIF3 alpha) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

    Fig2: Sample:; Lung (Mouse) Lysate at 40 ug; Primary: Anti-HIF3 alpha at 1/500 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 74 kD; Observed band size: 75 kD

    Fig2: Sample:; Lung (Mouse) Lysate at 40 ug; Primary: Anti-HIF3 alpha at 1/500 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 74 kD; Observed band size: 75 kD

    Fig3: Blank control (blue line): A549 (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).; Primary Antibody (green line): Rabbit Anti-HIF3 alpha antibody (bs-5898R),ilution: 1μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG .; Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.

    Fig3: Blank control (blue line): A549 (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).; Primary Antibody (green line): Rabbit Anti-HIF3 alpha antibody (bs-5898R),ilution: 1μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG .; Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.

  • Background
    Q9Y2N7

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"