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Anti-H2BC12 antibody

Cat.#: 176525

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Special Price 258.1 USD

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Product Information

  • Product Name
    Anti-H2BC12 antibody
  • Documents
  • Description
    Rabbit monoclonal antibody to H2BC12
  • Tested applications
    WB, ICC/IF, IHC-P, IP, FC
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    H2BK antibody; H2B/S antibody; H2BFT antibody; H2BFAiii antibody; HIST1H2BK antibody
  • Isotype
    IgG
  • Preparation
    This antigen of this antibody was recombinant protein
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:1,000

    ICC/IF: 1:100-1:500

    IHC-P: 1:100-1:500

    FC: 1:50-1:100

  • Validations

    Fig1: ICC staining Histone H2B(acetyl K20) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig1: ICC staining Histone H2B(acetyl K20) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig2: ICC staining Histone H2B(acetyl K20) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig2: ICC staining Histone H2B(acetyl K20) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining Histone H2B(acetyl K20) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining Histone H2B(acetyl K20) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B(acetyl K20) antibody. Counter stained with hematoxylin.

    Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B(acetyl K20) antibody. Counter stained with hematoxylin.

    Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Histone H2B(acetyl K20) antibody. Counter stained with hematoxylin.

    Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Histone H2B(acetyl K20) antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Histone H2B(acetyl K20) antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Histone H2B(acetyl K20) antibody. Counter stained with hematoxylin.

    Fig7: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2B(acetyl K20) antibody. Counter stained with hematoxylin.

    Fig7: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2B(acetyl K20) antibody. Counter stained with hematoxylin.

    Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Histone H2B(acetyl K20) antibody. Counter stained with hematoxylin.

    Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Histone H2B(acetyl K20) antibody. Counter stained with hematoxylin.

    Fig9: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H2B(acetyl K20) antibody. Counter stained with hematoxylin.

    Fig9: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H2B(acetyl K20) antibody. Counter stained with hematoxylin.

    Fig10: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Histone H2B(acetyl K20) antibody. Counter stained with hematoxylin.

    Fig10: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Histone H2B(acetyl K20) antibody. Counter stained with hematoxylin.

    Fig11: Flow cytometric analysis of Hela cells with Histone H2B(acetyl K20) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was use

    Fig11: Flow cytometric analysis of Hela cells with Histone H2B(acetyl K20) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was use

  • Background
  • References
    • Zhang B et al. An integrated peptide-antigen microarray on plasmonic gold films for sensitive human antibody profiling. PLoS One 8:e71043 (2013).
    • Price JV et al. On silico peptide microarrays for high-resolution mapping of antibody epitopes and diverse protein-protein interactions. Nat Med : (2012).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"